Dna2
5’ flap endonuclease and helicase involved in processing Okazaki fragments
Synonyms |
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Molecular weight |
Sc 172kDa
Sp 116kDa
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Biochemical properties |
Sc • Helicase/DNA dependent ATPase/exonuclease (1) •Helicase 5’ to 3’ (2) stimulated by RNA at the end of the DNA.
Xenopus •helicase reported less active than yeast in vitro (3)
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Motifs |
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Protein interactions |
Sc • Fen1 (1) • Tor (genetic ) (4) • Ctf4 (genetic) (5) DNA2 partly degraded in cells lacking ctf4. •Rpa (genetic) (6) and direct (7)
Sp • Cdc24 genetic (8)
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Modifications |
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Structure |
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Cellular location and expression |
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Other comments |
Sc •Associates with telomeres via sir3 (9) •some effect on lifespan (10)
Xl •can complement yeast mutant (3)
Dm
Mammals •Human homologues (11) (12)
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Revised by |
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Last edited |
13 April 08 |
1. Budd,M.E. and Campbell,J.L. (1995) A yeast gene required for DNA replication encodes a protein with homology to DNA helicases. Proc Natl Acad Sci U S A 92, 7642-7646.
2. Bae,S.H. and Seo,Y.S. (2000) Characterization of the enzymatic properties of the yeast dna2 Helicase/endonuclease suggests a new model for Okazaki fragment processing. J Biol Chem 275, 38022-38031.
3. Liu,Q., Choe,W. and Campbell,J.L. (2000) Identification of the Xenopus laevis homolog of Saccharomyces cerevisiae DNA2 and its role in DNA replication. J Biol Chem 275, 1615-1624.
4. Fiorentino,D.F. and Crabtree,G.R. (1997) Characterization of Saccharomyces cerevisiae dna2 mutants suggests a role for the helicase late in S phase. Mol Biol Cell 8, 2519-2537.
5. Formosa,T. and Nittis,T. (1999) Dna2 mutants reveal interactions with Dna polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full Dna2 helicase activity is not essential for growth. Genetics 151, 1459-1470.
6. Bae,S.H., Kim,J.A., Choi,E., Lee,K.H., Kang,H.Y., Kim,H.D., Kim,J.H., Bae,K.H., Cho,Y., Park,C. and Seo,Y.S. (2001) Tripartite structure of Saccharomyces cerevisiae Dna2 helicase/endonuclease. Nucleic Acids Res 29, 3069-3079.
7. Bae,K.H., Kim,H.S., Bae,S.H., Kang,H.Y., Brill,S. and Seo,Y.S. (2003) Bimodal interaction between replication-protein A and Dna2 is critical for Dna2 function both in vivo and in vitro. Nucleic Acids Res 31, 3006-3015.
8. Tanaka,H., Tanaka,K., Murakami,H. and Okayama,H. (1999) Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion. Mol Cell Biol 19, 1038-1048.
9. Choe,W., Budd,M., Imamura,O., Hoopes,L. and Campbell,J.L. (2002) Dynamic localization of an Okazaki fragment processing protein suggests a novel role in telomere replication. Mol Cell Biol 22, 4202-4217.
10. Hoopes,L.L., Budd,M., Choe,W., Weitao,T. and Campbell,J.L. (2002) Mutations in DNA replication genes reduce yeast life span. Mol Cell Biol 22, 4136-4146.
11. Masuda-Sasa,T., Imamura,O. and Campbell,J.L. (2006) Biochemical analysis of human Dna2. Nucleic Acids Res 34, 1865-1875.
12. Kim,J.H., Kim,H.D., Ryu,G.H., Kim,D.H., Hurwitz,J. and Seo,Y.S. (2006) Isolation of human Dna2 endonuclease and characterization of its enzymatic properties. Nucleic Acids Res 34, 1854-1864.