Mcm8

 

Related to Mcm2-7 family but apparently in distinct complex. Suggested involvement in elongation step of DNA replication (although conflicting reports). Absent in Sc and Sp.

 

Orthologues	DmRec
Molecular weight
Biochemical properties	•helicase (1) •ATPase (DNA dependent) (1)
Motifs	•Walker ATPase (1)
Interactions	Mammals •Orc2 and Cdc6 (2) •Mcm2-7 (4) , but note that this differs from (3) and (1)   latter in Xl
Modifications
Structure
Cellular location and expression	Xl • localises BrdU spots (1) •Loads after other MCMs in cell cycle (1)   Mammals •Loads after other MCMs in cell cycle (3) • cell location {Kinoshita et al., 2008, Microsc Res Tech, 71, 288-97}
Other comments	• (1) – (Xl in vitro extracts) suggest function in elongation Vs (2) (RNAi mammalian cells) suggest role in preRC formation (Cdc6 recruitment to chromatin)    •Some eukaryotes have a related protein Mcm9   Dm • recombination phenotype/ role in meiosis (5) (6)   Mammals •controlled by e2f1-4 (7)
Revised by
Last edited	13 July 09

 

 

1.    Maiorano,D., Cuvier,O., Danis,E. and Mechali,M. (2005) MCM8 is an MCM2-7-related protein that functions as a DNA helicase during replication elongation and not initiation. Cell 120, 315-328.

2.    Volkening,M. and Hoffmann,I. (2005) Involvement of human MCM8 in prereplication complex assembly by recruiting hcdc6 to chromatin. Mol Cell Biol 25, 1560-1568.

3.    Gozuacik,D., Chami,M., Lagorce,D., Faivre,J., Murakami,Y., Poch,O., Biermann,E., Knippers,R., Brechot,C. and Paterlini-Brechot,P. (2003) Identification and functional characterization of a new member of the human Mcm protein family: hMcm8. Nucleic Acids Res 31, 570-579.

4.    Johnson,E.M., Kinoshita,Y. and Daniel,D.C. (2003) A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13. Nucleic Acids Res 31, 2915-2925.

5.    Matsubayashi,H. and Yamamoto,M.T. (2003) REC, a new member of the MCM-related protein family, is required for meiotic recombination in Drosophila. Genes Genet Syst 78, 363-371.

6.    Blanton,H.L., Radford,S.J., McMahan,S., Kearney,H.M., Ibrahim,J.G. and Sekelsky,J. (2005) REC, Drosophila MCM8, drives formation of meiotic crossovers. PLoS Genet 1, e40.

7.    Hayashi,R., Goto,Y., Haga,A., Kobayashi,D., Ikeda,R. and Yoshida,K. (2006) Comparative genomics on MCM8 orthologous genes reveals the transcriptional regulation by transcription factor E2F. Gene 367, 126-134.