Pif1
DNA helicase (5’-3’) that may help to promote replisome progression in some regions of the genome such as the rRNA genes. Related to Rrm3.
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Synonyms |
SpPfh1 |
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Molecular weight |
98 kDa |
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Biochemical properties |
DNA helicase prefers to unwind RNA:DNA hybrids than DNA:DNA (1) |
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Motifs |
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Protein interactions |
Sc •Cac1 (2) •recQ (sgs1) suppresses (3)
Mammals •Telomerase (4)
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Modifications |
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Structure |
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Cellular location and expression |
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Other comments |
Sc • Suggested role in rDNA replication –less recombination (popped out circles) if lost (5) opposing effect to rrm3 • role in telomere maintenance and (2) reduces processivity of telomerase (6) •Suggested role with DNA2 in okazaki fragment synthesis (7)
Sp • Essential, post initiation defect in DNA replication? Suggest some role in replication (8)
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Revised by |
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Last edited |
15 April 08 |
1. Boule,J.B. and Zakian,V.A. (2007) The yeast Pif1p DNA helicase preferentially unwinds RNA DNA substrates. Nucleic Acids Res 35, 5809-5818.
2. Bessler,J.B., Torredagger,J.Z. and Zakian,V.A. (2001) The Pif1p subfamily of helicases: region-specific DNA helicases? Trends Cell Biol 11, 60-65.
3. Wagner,M., Price,G. and Rothstein,R. (2006) The absence of Top3 reveals an interaction between the Sgs1 and Pif1 DNA helicases in Saccharomyces cerevisiae. Genetics 174, 555-573.
4. Mateyak,M.K. and Zakian,V.A. (2006) Human PIF helicase is cell cycle regulated and associates with telomerase. Cell Cycle 5, 2796-2804.
5. Ivessa,A.S., Zhou,J.Q. and Zakian,V.A. (2000) The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. Cell 100, 479-489.
6. Boule,J.B., Vega,L.R. and Zakian,V.A. (2005) The yeast Pif1p helicase removes telomerase from telomeric DNA. Nature 438, 57-61.
7. Budd,M.E., Reis,C.C., Smith,S., Myung,K. and Campbell,J.L. (2006) Evidence suggesting that Pif1 helicase functions in DNA replication with the Dna2 helicase/nuclease and DNA polymerase delta. Mol Cell Biol 26, 2490-2500.
8. Zhou,J.Q., Qi,H., Schulz,V.P., Mateyak,M.K., Monson,E.K. and Zakian,V.A. (2002) Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases. Mol Biol Cell13, 2180-2191.