Sld2
Functions in initiation of replication. Key substrate of CDK, phosphorylation promotes interaction with Dpb11, required for initiation of replication in S. cerevisiae (1) (2)
Synonyms |
•DRC1 (SC) (3) •SpDrc1 Noguchi cb 12 599 (2); •RecQ4/RecQL4 in vertebrates may be orthologue as it has some homology but also has additional recQ related domains (4) (5) |
Molecular weight |
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Biochemical properties |
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Motifs |
Sc •Cdk sites (6) (7) • dephosphorylated by cdc14 (8) Sp •Cdc2 sites
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Protein interactions |
Sc •Dpb11 (3) (9) (direct) (6) (1) (2)
Sp •Cdc13/cig2 (10) •Cut5 through BRCT domains (10)
Xl •Cut5 (5) |
Modifications |
•Phosphorylation (6) (1) (2)
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Structure |
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Cellular location and expression |
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Other comments |
Sp • order loading relative to GINS, sld3 cdc45 (11)
Xl •Associates with chromatin after pre-RC formation. Required for chromatin association of replicative DNA polymerases (4) (5) •essential for replication in extracts (4)
Mammals • recQ4 involved in Rothmann-Thompson syndrome (4) • possible involvement in repair (12) |
Revised by |
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Last edited |
13 April 08 |
1. Zegerman,P. and Diffley,J.F. (2007) Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast. Nature 445, 281-285.
2. Tanaka,S., Umemori,T., Hirai,K., Muramatsu,S., Kamimura,Y. and Araki,H. (2007) CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Nature 445, 328-332.
3. Wang,H. and Elledge,S.J. (1999) DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96, 3824-3829.
4. Sangrithi,M.N., Bernal,J.A., Madine,M., Philpott,A., Lee,J., Dunphy,W.G. and Venkitaraman,A.R. (2005) Initiation of DNA replication requires the RECQL4 protein mutated in Rothmund-Thomson syndrome. Cell 121, 887-898.
5. Matsuno,K., Kumano,M., Kubota,Y., Hashimoto,Y. and Takisawa,H. (2006) The N-terminal noncatalytic region of Xenopus RecQ4 is required for chromatin binding of DNA polymerase alpha in the initiation of DNA replication. Mol Cell Biol 26, 4843-4852.
6. Masumoto,H., Muramatsu,S., Kamimura,Y. and Araki,H. (2002) S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast. Nature 415, 651-655.
7. Tak,Y.S., Tanaka,Y., Endo,S., Kamimura,Y. and Araki,H. (2006) A CDK-catalysed regulatory phosphorylation for formation of the DNA replication complex Sld2-Dpb11. EMBO J 25, 1987-1996.
8. Bloom,J. and Cross,F.R. (2007) Novel role for Cdc14 sequestration: Cdc14 dephosphorylates factors that promote DNA replication. Mol Cell Biol 27, 842-853.
9. Kamimura,Y., Masumoto,H., Sugino,A. and Araki,H. (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18, 6102-6109.
10. Noguchi,E., Shanahan,P., Noguchi,C. and Russell,P. (2002) CDK phosphorylation of Drc1 regulates DNA replication in fission yeast. Curr Biol 12, 599-605.
11. Yabuuchi,H., Yamada,Y., Uchida,T., Sunathvanichkul,T., Nakagawa,T. and Masukata,H. (2006) Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins. EMBO J 25, 4663-4674.
12. Kumata,Y., Tada,S., Yamanada,Y., Tsuyama,T., Kobayashi,T., Dong,Y.P., Ikegami,K., Murofushi,H., Seki,M. and Enomoto,T. (2007) Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts. Biochim Biophys Acta 1773, 556-564.